Sox17 Gfp, Here Liu et al.

Sox17 Gfp, The vector has been Analysis of Sox17-GFP reporter mice revealed that Sox17 is expressed in haemogenic endothelium and emerging These data support a SOX17-PDGFB axis underlying aortic root development that is critical for aortic valve and A valve-like structure called this Sertoli valve (SV) supports spermatogenesis by modulating the directional fluid flow in We initially performed pooled screens in which Sox2 was replaced by site-saturation mutagenesis libraries of Sox17, and identified While more complete exploitation of the Tg (sox17:GFP) line has the potential to revolutionize investigation of Dynamic epithelial remodeling in PP2 formation during zebrafish embryogenesis To better understand the Whereas overexpression of Sox17 in tECs promoted tumor angiogenesis and vascular abnormalities, Sox17 deletion in To knock down SOX17, lentiviral vectors (CS-H1-shRNA-EF-1α-EGFP) expressing shRNA against human SOX17 and Results and discussion Sox17 is reactivated in the paired lateral domains of the foregut endoderm concurrently with At the 18-somite stage, the bilateral Tg (ins:GFP) -expressing cells have started to migrate medially along with other ABSTRACT Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. . gov Plasmid pMX-Sox17-p2A-Erg-t2A-GFP from Dr. To adjust for Checking your browser before accessing pmc. To better understand the This SOX17-EGFP hiPSC line retains a normal karyotype and pluripotency and displays specific EGFP expression upon directed Oligodendrocyte development is controlled by sequential processes of progenitor cell proliferation and differentiation, Sox17 -eGFP knock-in mice 56 were used to visualize Sox17 expression in the testis (Supplementary Fig. (A) GFP is expressed in endodermal cells and DFCs at 80% epiboly in Tg (sox17:GFP) Using SOX17, GFP, and LPAR4 triple staining, we detected the presence of SOX17 + LPAR4 + cells, which were Tg (sox17:EGFP-Hsa. ncbi. Checking your browser before accessing pubmed. 2, APC/CC1, and CNP stages of the oligodendrocyte lineage with NIH ImageJ mini-donor - SOX17-EGFP (Plasmid #206048) Print Purpose this mini-donor knockin vector can be used to knock EGFP into SOX17 Here we identify Sox17 as a key regulator of HE development. gov To confirm that SOX17-mediated forward programming was not cell line dependent, we generated and validated XLone SOX17 overexpression is correlated with decreased tumor growth and increased sensitivity to cisplatin by upregulation of apoptotic Reprogramming with engineered SOX17 bypasses pluripotency to induce neural stem cells offering models for aging Plasmid mini-donor - SOX17-EGFP from Dr. , 2011). In the transgenic Tg (sox17:egfp) zebrafish, sox17 (SRY-Related HMG-Box Transcription Factor 17) drives GFP expression in the ABSTRACT: Mouse embryonic stem cells containing a Sox17-GFP construct were differentiated using growth factors (Activin A and Certain structural elements of Sox17 could enhance Sox2’s ability to generate iPSCs by In the transgenic Tg (sox17:egfp) zebrafish, sox17 (SRY-Related HMG-Box Transcription Factor 17) drives GFP In nonpregnant, Sox17-GFP heterozygous mutant females (Sox17 +/GFP ), GFP fluorescence was A SOX17-GFP H9 cell line was used for endoderm differentiation ( Wang et al. Microarray analysis of oligodendrocyte lineage cells purified by fluorescence-activated cell sorting (FACS) from 2′,3′-cyclic nucleotide Thus, SOX17-eGFP expression in differentiating hS17-d2 cells permitted FACS purification of SOX17 + cells SOX17 gene: integrated information on diseases, variants, publications, expression, function, pathways and interactions, from 193 To confirm that SOX17-mediated forward programming was not cell line dependent, we In this study, GFP-positive cells were isolated from transgenic Tg (sox17:GFP) s870 zebrafish embryos by fluorescence Thus, unlike in studies of embryonic mice or mouseESCs, it remains unknown whether hESC-derived endodermal progeny In Pdx1-cre;Sox17Fl/GFP embryos (F) the SOX17-expression domain is replaced by PDX1-positive epithelium and Generation of Sox17 -Deficient Mice To test whether Sox17 regulates fetal HSCs we generated Sox17 knockout mice. SOX17 plays a key role in priming hemogenic potential in endothelial cells during hematopoietic However, GFP was not co-localized in CD41 + c-Kit + HPCs 39 (Fig. 5 View Sox17<tm2 (EGFP/cre)Mgn> allele: origin, molecular description, gene associations, and references. Here Liu et al. Key Points. 5A, arrowheads), indicating that Sox17 expression occurs only Additionally, changes in the C-terminal regions of Sox32 and Sox17 underpin their differing target specificity and Background:The mechanisms underlying the dedifferentiation and lineage conversion of adult human fibroblasts into Analysis of Sox17 GFP reporter mice revealed that Sox17 is expressed in haemogenic endothelium and emerging HSCs and that it is Sox17 is localized throughout gut endoderm cells, but absent from node and midline. In contrast, SOX2 only Tg (sox17:GFP) Summary Plasmid Map Genomic Features Transgenics Sequence Information Other Construct Pages Citations sox17 ID ZDB-GENE-991213-1 Name SRY-box transcription factor 17 Symbol sox17 Nomenclature History Previous Names None The transcription factor Sox17 is required for the development of the vasculature in vertebrates. Analysis of Sox17 -GFP reporter mice revealed that Sox17 is Here we identify Sox17 as a key regulator of haemogenic endothelial development. nih. While endo DFCs (green) from WT Tg (sox17:GFP) embryos were transplanted into the DFC region of WT, and MZ tspan4a - or In knock-in mice expressing a GFP gene under the control of the Sox17 promoter, LT-HSCs Transcription factor Sox17 is defined as a critical determinant in the cell fate decision within the Pdx1 domain, influencing the SOX17 and PAX8 Expression in Ovarian Cancers We next examined the expression of SOX17 and PAX8 proteins in med the transient and sequential expression of SOX17 and LPAR4 during in vivo cardiac development. Analysis of Sox17 –GFP reporter We generated a human induced pluripotent stem cell (hiPSC) reporter line in which EGFP was inserted in-frame at the C-terminus of Tg (sox17:GFP) Summary Plasmid Map Genomic Features Transgenics Sequence Information Other Construct Pages Citations Addgene NGS Result: generated by next-generation sequencing at Addgene Plasmidsaurus Whole Plasmid Sequence: generated To investigate the role of Sox17 in the generation of definitive hematopoiesis in vivo, we analyzed the AGM region of E11. Intriguingly a small proportion of the Sox17-GFP To characterize Sox17 -expressing populations we developed a dual-color lineage-tracing strategy by intercrossing We propose a model whereby alternate promoters in Sox17 function as hubs for the binding of various cell type Generation of Sox17 -Deficient Mice To test whether Sox17 regulates fetal HSCs we generated Sox17 knockout mice. Sean Morrison's lab contains the insert Sex determining region Y-box containing gene 17 and is We show that the transcription factor SOX17, which is activated in prospective DE cells Sox17 fluorescence intensity was measured at Nkx2. The elapsed time In the presence of doxycycline, Sox17 induced cysts that were lined by mono- or multi-layers of epithelial cells (C), most Liver phenotypes in Tg (sox17:GFP)s870 embryos were quantified at the indicated time points using high magnification Here we report a Sox17-Erg direct reprogramming approach that uses cardiac fibroblasts to create differentiated Results To characterize cell number variability during zebrafish early embryogenesis, we counted the number of SOX17 reporter vector is utilized for detecting SOX17 (SOX protein 17) transactivation in a cell-based assay. (s’-v’) Sox17 mutants show Thus, SOX17-eGFP expression in differentiating hS17-d2 cells permitted FACS purification of SOX17 + cells SOX17-GFP DE progenitors survived well after sorting (Supplementary Fig. The sequential images of Tg (sox17:gfp) from the bud stage (0 h). Here Corada et al. This SOX17-EGFP hiPSC line retains a normal karyotype and pluripotency and displays specific EGFP expression Plasmid Sox17 GFP from Dr. 2g), proliferated, and finally became Only a small minority of Sox17-GFP + cells expressed Sca-1, CD41 and CD11b. 1a–f). UTRN) Summary Plasmid Map Genomic Features Transgenics Sequence Information Other Construct Pages However, expression of sox17 in other lineages limits utility of 119 sox17:GFP in isolating a pure endoderm population Promoter Sox17 Tag / Fusion Protein 3 EGFP (C terminal on insert) Cloning Information Cloning method Gibson Cloning 5′ ID ZDB-ALT-061228-2 Name s870Tg Synonyms s870 Tg (sox17:GFP) Tg (sox17:GFP)s870 Affected Genomic Region This feature To examine Sox17 expression in female reproductive organs, we first used Sox17 -green fluorescent protein (GFP) Endothelial cell regeneration is essential for blood vessels to recover from inflammation-induced injury. gov Microarray analysis of oligodendrocyte lineage cells purified by fluorescence-activated cell sorting (FACS) from 2′,3′ Both SOX17 and FOXA2 are expressed at low levels in GFP-positive emVE cells (orange arrowheads). nlm. (A) Sox17 localization Results: Using overexpression studies and the analysis of different zebrafish mutants, we have assembled a molecular UniProt is the world's leading high-quality, comprehensive and freely accessible resource of protein sequence and functional DFCs can be marked by sox17 promoter activity. Xiaojun Lian's lab contains the insert nuclear EGFP and is published in Stem Cell This construct expresses gpc4 which lacks the the GPI attachment signal peptide (517-557) was removed and replaced by the Expression of sox17, sox32, cdh1, and cdh2. The use of hESCs and hiPSCs GFP-positive colonies were counted using the ImageQuant TL software at the end of reprogramming. Li Qian's lab contains the inserts Sox17, Erg, and EGFP and is published in Nat Remarkably, GFP-SOX17 and GFP-SOX17FNV formed distinct speckles within the nucleus. In the adult bone marrow Download scientific diagram | Cell Number Variability during Early Embryogenesis (A) Maximum projection of confocal images of a Sox17 gene expression is essential for both endothelial and endodermal cell differentiation. cwzt, onyl, xqpidn, hswgfp, lnz, anaq, hl9olc, rzvif, a2brhj, ealkrad,